Well, ThumbsPlus has never supported .ai files internally, but you may have loaded those Illustrator files either through OLE (if Illustrator supports Windows' "Object Linking and Embedding" method) or through the Ghostscript PDF plug-in (which can read some but not all .ai files).
If you still have your older (working with .ai) copy of ThumbsPlus installed: Run it, right-click on an .ai file, pick "Configure File Type" and see what it says for "Load File Using"..:
[*]If it's OLE, the field to the right will probably show the name of the application that is actually reading the file and passing it on to ThumbsPlus. You can then launch ThumbsPlus 9 and do the same thing to replicate this setting there.
[*]If it's Cerious Plug-in, you'll first have to get the PDF plug-in to work with ThumbsPlus 9 on your system. See the [msg=2950]2nd message on this page[/msg] for instructions. Then right-click on an .ai file, pick the Configure File Type option again and set Load Using to "Cerious Plug-in" and the field at its right to "plug_gs.tpp".
[*]If it's something else, try to duplicate that in ThumbsPlus 9 and if that doesn't work, let me know what it was :)
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If you no longer have your old ThumbsPlus installed, you could just try these loading methods for .ai files in ThumbsPlus 9 to see which one works. I'd start with the OLE option because it should be the easiest and most accurate, if it's available on your system.